rotating rod apparatus  (BIOSEB Inc)

Journal: bioRxiv

Article Title: Preventing Microglial Reactivity Protects from Acute and Progressive Neuronal Dysfunction, Motor Impairments and Sedation following Alcohol Abuse

doi: 10.1101/2025.06.12.658437

Figure Lengend Snippet: a. Representative epifluorescence microscopy images of NeuN-labeled neurons in somatosensory cortex of saline- and ethanol-treated Cx3cr1 GFP/+ and Cx3cr1cre Tg/0 ;MyD88 F/F ;Rosa26 tdTomato mice after 10 consecutive days of treatment. Scale bar, 100 µm. b. Quantification of NeuN + neurons per field of view (FOV) in somatosensory cortex of mice treated as described in ( a ). n = 4-6 mice per group. Two-way ANOVA with Tukey’s multiple comparison test. c. Representative epifluorescence microscopy images of NeuN-labeled neurons in prefrontal cortex of saline- and ethanol-treated MyD88 -/- , MyD88 F/F , and Cx3cr1cre Tg/0 ;MyD88 F/F mice after 10 days of treatment. Scale bar, 100 µm. d. Quantification of NeuN + neurons per FOV in prefrontal cortex of mice treated as described in ( c ). n = 5 mice per group. e. Rotarod assessment of ethanol-induced motor impairments following a single binge ethanol exposure (2 g/kg BW). Number of trials to stay on the rod and latency to falling off the rotarod for ethanol-treated MyD88 F/F (n=5) and Cx3cr1cre Tg/0 ;MyD88 F/F (n=9) mice. Unpaired t-test and Wilcoxon matched-pairs signed rank test, respectively. f. Quantification of blood ethanol levels in plasma samples collected on day 3 of binge ethanol exposure (5 g/kg BW) from MyD88 F/F and Cx3cr1cre Tg/0 ;MyD88 F/F mice. n = 5–6 mice per group. Unpaired t-test. g. Representative confocal microscopy images of Iba1-labeled microglia in prefrontal cortex of mice treated as described in ( c ) on day 10 of binge ethanol exposure. Scale bar, 10 µm. h, i. Sholl analysis (h) and quantification of microglial process volume, process length and branch points (i) in prefrontal cortex of mice treated as described in ( c ). n = 5 mice per group. Data presented as mean ± SEM. d, i: One-way ANOVA with Tukey’s multiple comparison test; h: Two-way ANOVA with Tukey’s multiple comparison test. Only statistically significant comparisons are shown.

Article Snippet: Mice were placed on a non-accelerating rotating rod (fixed speed at 16 rpm, 3 cm diameter rod, 44.5 cm height, Rotamex-5; Columbus Instruments).

Techniques: Epifluorescence Microscopy, Labeling, Saline, Comparison, Clinical Proteomics, Confocal Microscopy

Journal: bioRxiv

Article Title: Preventing Microglial Reactivity Protects from Acute and Progressive Neuronal Dysfunction, Motor Impairments and Sedation following Alcohol Abuse

doi: 10.1101/2025.06.12.658437

Figure Lengend Snippet: a. Schematic diagram of experimental design for the assessment of ethanol-induced motor impairments. (i) rotarod and locomotor activity cage assessments performed after the first and second day of binge ethanol oral administration (2 g/kg BW). (ii) Loss of righting reflex (LORR) assessment after a single intraperitoneal injection of 3.5 g EtOH/kg BW. b. Number of trials to stay on the rod and latency to falling off the rotarod on day 1 of the 10-day ethanol course. n = 19 ethanol-treated Cx3cr1 GFP/+ , n = 21 Cx3cr1 creERT2 ;MyD88 F/F and n = 14 Tmem119 creERT2 ;MyD88 F/F mice. One-way ANOVA with Tukey’s multiple comparison test (trials to stay), and Friedman with Dunn’s multiple comparisons test (latency to fall). c. Total beam breaks recorded in the locomotor activity cage over 2 hours after ethanol administration on day 2 of the 10-day ethanol course. Saline control data were collected one day before ethanol course initiation, as described in ( a ( i )). Data binned every 5 minutes. n = 5 Cx3cr1 GFP/+ , n = 9 Cx3cr1 creERT2 ;MyD88 F/F , n = 8 Tmem119 creERT2 ;MyD88 F/F mice. Kruskal-Wallis with Dunn’s multiple comparisons test. d. Latency to LORR and duration of LORR following treatment as described in ( a(ii) ). n = 9 MyD88 F/F and n = 13 Tmem119 creERT2 ;MyD88 F/F mice. Unpaired t-test. e. Quantification of blood ethanol concentration in plasma samples on day 3 and 10 of binge ethanol exposure (2g/kg BW, n = 7-8 mice / group). One-way ANOVA with Tukey’s multiple comparison test. f. Quantification of blood ethanol concentration in plasma samples on day 1 of binge ethanol exposure (5g/kg BW). n = 8 mice/group. Unpaired t-test. Data presented as mean ± SEM. Only statistically significant comparisons are shown.

Article Snippet: Mice were placed on a non-accelerating rotating rod (fixed speed at 16 rpm, 3 cm diameter rod, 44.5 cm height, Rotamex-5; Columbus Instruments).

Techniques: Activity Assay, Injection, Comparison, Saline, Control, Concentration Assay, Clinical Proteomics